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目的 在三叉神经主核 (PrV)上检测不同剂量甘丙肽拮抗剂M 35和M 4 0对甘丙肽外向电流的作用。方法 全细胞膜片钳记录技术。结果 0 .0 1、0 .0 3及 0 .1μmol·L- 1的M 35 ,使甘丙肽引发的外向电流分别抑制 5 3.1%、 4 0 .4 %及 2 7.2 % ;但 1、3μmol·L- 1M 35使甘丙肽外向电流分别增大16 .2 %及 38.4 %。而给予 0 .0 1及 0 .0 3μmol·L- 1M 4 0时 ,甘丙肽外向电流增大 93.7%及 6 8.4 % ;0 .3、1及 3μmol·L- 1M 4 0使甘丙肽外向电流分别抑制 4 9.2 %、6 9.9%及 87.2 %。结论 低浓度M 35为甘丙肽拮抗剂 ,高浓度M 35有甘丙肽激动剂作用 ;低浓度M 4 0为甘丙肽激动剂 ,高浓度M 4 0为甘丙肽拮抗剂 ,PrV同一神经元上可能存在不同甘丙肽亚型受体
Objective To detect the effects of galanin antagonists M 35 and M 40 on the outward current of galanin in the main trigeminal nerve (PrV). Methods Whole-cell patch-clamp recording technique. The results showed that the outward currents induced by galanin were inhibited by 5.01%, 4.04% and 7.2%, respectively, but Mμm at 0.1 μmol·L -1 and 0.1 μmol·L -1 L-1M 35 increased the outward current of galanin by 16.2% and 38.4% respectively. However, the outward current of galanin increased by 93.7% and 6 8.4% at 0. 01 and 0. 3 μmol·L-1M 4 0, respectively. When 0.3, 1, Outward currents were suppressed by 4 9.2%, 69.9% and 87.2% respectively. Conclusions Low M 35 is a galanin antagonist, high concentration M 35 is a galanin agonist, low M 4 0 is a galanin agonist, high M 4 O is a galanin antagonist, and PrV is the same There may be different galanin subtype receptors on neurons