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为了解桂林市HIV-1感染者中HIV-1流行毒株亚型的分子流行病学特征,采集桂林市HIV-1感染者的抗凝全血样品42份,分离PBMC并提取DNA,用巢氏PCR方法扩增病毒env基因,并进行序列测定和亚型分析。系统进化分析表明,桂林市HIV-1感染者样品分别属于4亚型:重组型CRF01-AE亚型4份、重组型CRF08-BC亚型15份、重组型CRF07-BC亚型8份和01C亚型3份。可见,桂林市HIV-1感染者中流行毒株主要为CRF-BC,应加强对HIV-1毒株亚型变异的监测,及时制定新的防治策略。
In order to understand the molecular epidemiological characteristics of HIV-1 pandemic strain among HIV-1 infected persons in Guilin, 42 samples of anticoagulated whole blood from HIV-1 infected persons in Guilin were collected and PBMCs were isolated and DNA extracted. The env gene was amplified by PCR and sequenced and subtype analyzed. Phylogenetic analysis showed that samples from HIV-1 infected patients in Guilin belong to 4 subtypes: 4 copies of recombinant CRF01-AE, 15 copies of recombinant CRF08-BC, 8 copies of recombinant CRF07-BC, Subtype 3 copies. It can be seen that the predominant strains of HIV-1 infected persons in Guilin are mainly CRF-BC. The monitoring of subtypes of HIV-1 strains should be strengthened and new prevention and treatment strategies should be formulated in time.