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目的了解宁波地区食品中致病菌检出情况和菌株的耐药性,发现其流行优势菌。方法致病菌检测采用直接分离与增菌分离相结合的方法;细菌鉴定采用生化筛检和API等方法;细菌分型采用诊断血清和PFGE基因分型;药敏试验采用K-B法,耐药基因检测采用PCR法。结果 6 812份食品样品中检出目标菌7类12种,共2 331份,检出率为34.22%,致病性弧菌检出数最高,其次为沙门菌和致病性气单胞菌。副溶血性弧菌与其他致病菌差异有统计学意义(P<0.01),分离出10个血清群和29个PFGE型,其中O6、O5血清群和PFGE 1型是副溶血性弧菌的主要优势流行型。检出的致病菌对大多数抗生素敏感,其中3株气单胞菌为带aacc耐药基因的多重耐药菌。结论宁波地区食品中致病菌种类较多,易引起食源性疾病;各类致病菌均有流行优势株,副溶血性弧菌是最主要的流行优势株;血清分型和PFGE型能发现优势菌,但均有一定的局限性。
Objective To understand the detection of pathogenic bacteria in food in Ningbo and strains of drug resistance, found that its prevalence of bacteria. Methods The pathogenic bacteria were detected by the method of direct separation combined with enrichment and separation, the methods of biochemical screening and API were used for bacterial identification, the genotyping of bacteria by using the method of genotyping and PFGE, the KB method and drug resistance gene Detection using PCR method. Results Among 6 812 food samples, 12 kinds of 12 kinds of 7 kinds of target bacteria were detected, of which 2 331 were detected, the detection rate was 34.22%, the highest number of pathogenic Vibrio was detected, followed by Salmonella and pathogenic Aeromonas bacteria . Vibrio parahaemolyticus was significantly different from other pathogens (P <0.01), 10 serogroups and 29 PFGE genotypes were isolated, of which O6 and O5 serogroups and PFGE 1 genotypes were Vibrio parahaemolyticus The main advantage of popular type. The pathogens detected were sensitive to most antibiotics, of which 3 Aeromonas were multi-drug resistant strains with aacc resistance gene. CONCLUSIONS Foodborne pathogens are more likely to cause food-borne diseases in Ningbo. Most of the pathogenic bacteria have prevailing strains. Vibrio parahaemolyticus is the predominant predominant strain. Serotyping and PFGE patterns Dominant bacteria found, but have some limitations.