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目的 观察低温对沙土鼠单侧脑缺血时氨基酸递质释放的影响。方法 予１８ 只雄性蒙古沙土鼠单侧颈内动脉钳夹３０ 分钟，随机分为常温（３７ ℃）和低温（３２ ℃）组，分别观察两组缺血侧海马部谷氨酸、甘氨酸和γ－氨基丁酸（ＧＡＢＡ）浓度变化。采用体内微透析和高效液相色谱法监测。结果 常温组脑缺血后谷氨酸和甘氨酸浓度明显升高，而低温组无明显上升；再灌注期常温组ＧＡＢＡ 浓度迅速下降，而低温组ＧＡＢＡ 长时维持在较高浓度。在缺血和再灌注期，低温组兴奋毒性指数显示低于常温组（Ｐ＜ ０．０１）。结论 低温的神经保护特性可能是由于细胞外兴奋毒性指数的降低所致。
Objective To observe the effect of hypothermia on the release of amino acid neurotransmitters in gerbils during unilateral cerebral ischemia. Methods Eighteen male Mongolian gerbils were fixed for 30 minutes in the unilateral internal carotid artery and were randomly divided into normal temperature (37 ℃) and low temperature (32 ℃) groups. Glutamate, glycine and γ - aminobutyric acid (GABA) concentration changes. In vivo microdialysis and high performance liquid chromatography monitoring. Results The concentrations of glutamic acid and glycine in normal temperature group increased obviously, but not in hypothermia group. GABA concentration in normal temperature group decreased rapidly at reperfusion stage, while GABA concentration maintained at higher concentration in hypothermia group. During ischemia and reperfusion, the excitotoxicity index in hypothermia group was lower than that in normal temperature group (P <0.01). Conclusion The neuroprotective properties at low temperature may be due to the decrease of extracellular excitotoxicity index.