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目的研究短穗兔耳草和短管兔耳草对急性酒精肝损伤的保护作用及其作用机制。方法将小鼠随机分为9组,每组10只,分别为正常组,模型组,联苯双酯组(150 mg·kg~(-1)),短穗兔耳草低、中、高剂量组(1.0,2.0,4.0 g·kg~(-1)),短管兔耳草低、中、高剂量组(1.0,2.0,4.0 g·kg~(-1))。采用灌胃50%乙醇的方法制备急性酒精肝损伤模型,连续灌胃给药7 d,于造模后16 h各组取血、取肝脏,测定血清中丙氨酸氨基转氨酶(ALT)、天门冬氨酸转移酶(AST)、总胆固醇(TC)、甘油三酯(TG)、肿瘤坏死因子-α(TNF-α)、核转录因子kappa B(NF-κB)各项指标,测定肝组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)、还原性谷胱甘肽(GSH)的含量,PCR检测肝组织中TNF-α相对表达量。结果与模型组比较,短穗兔耳草和短管兔耳草高剂量组可显著降低血清中ALT、AST、TC、TG含量可显著升高肝匀浆中SOD、GSH含量,并降低MDA含量,差异均有统计学意义(P<0.05,P<0.01);短穗兔耳草各剂量组和短管兔耳草高剂量组可显著降低TNF-α、NF-κB的含量,差异有统计学意义(P<0.05,P<0.01);短穗兔耳草和短管兔耳草高、中剂量组小鼠肝组织中TNF-α mRNA的相对表达量均显著降低,差异有统计学意义(P<0.01)。结论短穗兔耳草和短管兔耳草均具有抗急性酒精肝损伤作用,其作用与氧化应激及对炎症因子的调控相关。
Objective To study the protective effect and its mechanism of Acridothera anguillarum Methods The mice were randomly divided into 9 groups (n = 10): normal group, model group, bifendate group (150 mg · kg -1), low, middle and high (1.0,2.0,4.0 g · kg -1), short, low, medium and high dose groups (1.0, 2.0 and 4.0 g · kg -1) respectively. Acute alcoholic liver injury model was prepared by intragastric administration of 50% ethanol. After continuous intragastric administration for 7 days, the rats were sacrificed at 16 hours after model establishment, and the liver was taken for determination of serum ALT, AST, TC, TG, TNF-α and NF-κB in liver tissue were determined, and the changes of liver tissue The contents of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) in the homogenate were measured. The relative expression of TNF-α in the liver tissue was detected by PCR. Results Compared with the model group, the content of ALT, AST, TC and TG in the serums of H. spinosa and T. capricola high dose groups could significantly increase the content of SOD and GSH in liver homogenate and decrease the content of MDA , Respectively (P <0.05, P <0.01). The contents of TNF-α and NF-κB in each group and the short-tube group were significantly decreased (P <0.05, P <0.01). The relative expression of TNF-α mRNA in the liver tissue of high-dose and medium-dose group decreased significantly, the difference was statistically significant (P <0.01). CONCLUSIONS: Eriocheir sinensis and Eupatorium adenophorum both have anti-acute alcoholic liver injury, and their effects are related to oxidative stress and the regulation of inflammatory cytokines.