论文部分内容阅读
目的 :通过检测小儿血管瘤基质金属蛋白酶 (matrixmetalloproteinases ,MMPs)的表达 ,探讨其发病机理。方法 :取小儿血管瘤标本 6 0例 ,其中增殖组 30例 ,退化组 30例。另取 12例正常组织标本作为对照。用原位杂交方法观察血管瘤微血管内皮细胞中MMP - 1mRNA的表达。用免疫组化方法 (SABC法 )观察MMP - 9蛋白的表达。并通过图像分析技术进行定量研究。结果 :通过计数方法显示各组MMP - 1mRNA阳性表达率差异均无显著性 (P >0 .0 5 )。增生组MMP - 9蛋白阳性表达率明显高于退化组及正常组 ,差异有显著性意义 (P <0 .0 5 ,P <0 .0 5 )。图像分析结果显示MMP - 1mRNA阳性面积表达率各组之间差异均无显著性 (P>0 .0 5 )。增生组MMP - 9蛋白表达明显高于退化组及正常组 ,差异有显著性意义 (P <0 .0 5 ,P <0 .0 5 )。结论 :MMP - 9可能通过促进新生血管形成在血管瘤发病机制中具有重要作用。MMP - 1可能通过转录后调控促进血管瘤增生。
Objective: To explore the pathogenesis of matrix metalloproteinases (MMPs) in children by detecting the expression of matrix metalloproteinases (MMPs). Methods: Totally 60 children with hemangiomas were enrolled, including 30 cases of proliferative group and 30 cases of degenerative group. Another 12 cases of normal tissue samples as a control. The expression of MMP - 1 mRNA in vascular endothelial cells was observed by in situ hybridization. The expression of MMP - 9 protein was observed by immunohistochemistry (SABC method). And through image analysis technology for quantitative research. Results: The positive rates of MMP - 1 mRNA in each group were not significantly different by counting method (P> 0.05). The positive rate of MMP - 9 in hyperplasia group was significantly higher than that in degenerative group and normal group (P <0.05, P <0.05). The result of image analysis showed that the positive rate of MMP - 1 mRNA expression in each group had no significant difference (P> 0.05). The expression of MMP - 9 in hyperplasia group was significantly higher than that in degenerative group and normal group (P <0.05, P <0.05). Conclusion: MMP - 9 may play an important role in the pathogenesis of hemangiomas by promoting neovascularization. MMP - 1 may promote the proliferation of hemangiomas through post - transcriptional regulation.