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目的:探讨三碘甲腺原氨酸(T3)对大鼠成骨细胞增殖的影响。方法:采用骨组织块法原代分离培养新生SD大鼠颅骨成骨细胞,然后配置含不同浓度(10-7mol/L、10-8mol/L、10-9mol/L)T3的培养基,与大鼠成骨细胞共培养4d,采用噻唑蓝(MTT)法检测细胞增殖能力,采用细胞化学染色法测定成骨细胞ALP活性。结果:T3可促进大鼠成骨细胞增殖,并呈剂量依赖性;随T3浓度的增高,成骨细胞表达ALP活性增强(P<0.05)。结论:10-7mol/L～10-9mol/L浓度的T3可通过刺激成骨细胞的增殖,对预防和治疗骨质疏松发挥一定作用。
Objective: To investigate the effect of triiodothyronine (T3) on the proliferation of rat osteoblasts. Methods: Osteoblasts of newborn SD rats were primarily isolated and cultured by using bone tissue block method. Then medium containing different concentration of T3 (10-7mol / L, 10-8mol / L, 10-9mol / L) The rat osteoblasts were co-cultured for 4 days. The proliferation of osteoblasts was detected by MTT assay. The ALP activity of osteoblasts was determined by cytochemical staining. Results: T3 promoted the proliferation of osteoblasts in a dose-dependent manner. With the increase of T3 concentration, the activity of ALP in osteoblasts increased (P <0.05). Conclusion: The concentration of 10-7mol / L ~ 10-9mol / L of T3 may play an important role in prevention and treatment of osteoporosis by stimulating the proliferation of osteoblasts.