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目的:研制大鼠肺气虚证模型,为研究因慢性呼吸道感染导致肺气虚证的诊治提供可靠的实验动物。方法:健康SD大鼠随机分为空白对照组、烟熏对照组,以及高、中、低菌量模型组。模型组采用呼吸道生物被膜菌(bacterial biofilm,BF)铜绿假单胞菌滴鼻法,辅以寒冷疲劳刺激引起大鼠呼吸道慢性感染,诱导肺气虚证的发生。观察各组证候表现、游泳时间、体重变化、血液流变学指标的改变,以及肺支气管组织器官病理改变。结果:高、中、低菌量模型组大鼠的一般体征均符合肺气虚证,症状较烟熏组稳定。游泳时间和体重增加低于空白对照组,尤以高菌量组为明显(P<0.05,P<0.01)。血流变指标提示血液流变性有障碍,肺及支气管炎症明显,炎症的程度和感染菌量有关。结论:将呼吸道生物被膜菌(铜绿假单胞菌)以滴鼻法并辅以寒冷疲劳刺激,可以诱导大鼠肺气虚证模型,相关的指标表明证候表现与中医学理论的病机相符,且该模型具有稳固、易复制、贴近原型的特点。
Objective: To develop a model of lung qi deficiency syndrome in rats and provide a reliable experimental animal for the research of diagnosis and treatment of pulmonary qi deficiency syndrome caused by chronic respiratory infection. Methods: Healthy SD rats were randomly divided into blank control group, smoked control group, and high, medium and low bacterial model groups. The model group was treated with respiratory biofilm (Pseudomonas aeruginosa Pseudomonas aeruginosa nasal drops nasal drops method, supplemented by cold fatigue stimulation caused chronic respiratory tract infection in rats, induced pulmonary qi deficiency syndrome. Observed the performance of each group syndromes, swimming time, body weight changes, changes in hemorheology indicators, as well as lung bronchial tissue and organ pathology. Results: The general signs of high, medium and low bacteria model group were consistent with the deficiency of lung Qi, the symptoms were stable than the smoked group. Swimming time and weight gain were lower than those in the blank control group, especially in the high-bacteria group (P <0.05, P <0.01). Hemorheological indicators suggest that there is an obstacle to hemorrheology, lung and bronchial inflammation is obvious, the degree of inflammation and infection of the amount of bacteria. CONCLUSION: The model of lung-qi deficiency in rats can be induced by intranasal nasal biofilm (supplemented with Pseudomonas aeruginosa) and cold fatigue stimulation. The relevant indexes indicate that the syndromes are consistent with the pathogenesis of TCM, And the model has a stable, easy to copy, close to the prototype features.