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从土壤中分离筛选明串珠菌,通过对其生理生化特性的研究并基于16S rDNA序列的系统发育树分析确定其分类地位。结果该菌株为革兰氏阳性、过氧化氢酶阴性、精氨酸和七叶苷水解阴性、发酵葡萄糖产酸产气、不生成葡聚糖。可发酵利用果糖、蔗糖、蜜二糖、麦芽糖、L-阿拉伯糖、D-木糖、棉籽糖、核糖、水杨苷,延迟利用半乳糖和甘露糖,微弱利用纤维二糖、乳糖、海藻糖和L-鼠李糖,不可利用甘露醇;系统发育树表明该菌株属于假肠膜明串珠菌,命名为kimshi005,在GenBank上的登录号为GU130195。
Isolation and identification of Leuconostoccus fumigatus from the soil were studied by its physiological and biochemical characteristics and phylogenetic tree analysis based on the 16S rDNA sequence. Results The strain was Gram-positive, catalase-negative, arginine and aseptic negative hydrolysis, fermentation of glucose acid production of gas, does not produce dextran. Fermentative utilization of fructose, sucrose, melibiose, maltose, L-arabinose, D-xylose, raffinose, ribose, salicin, delayed use of galactose and mannose, faint use of cellobiose, lactose, trehalose And L-rhamnose, no mannitol was available. Phylogenetic tree indicated that the strain belonged to Leuconostoc mesenteroides, named kimshi005, and its accession number was GU130195 in GenBank.