目的建立固相萃取方法同时提取尿中的39种药毒物并用高效液相色谱法进行分析。方法以多沙普仑为内标,1ml尿样经Oasis小柱固相萃取,用HPLC进行分析。结果39种药毒物可同时从尿中提出,内源性物质不干扰测定。其绝对回收率除吗啡外均大于70％;天内及天间精密度均小于10％;检测限1-15ng／ml;线性相关系数在0.9977以上。结论本法快速、简便、重现性好,空白干扰小,可用于实际案例的药物毒物筛选。 Objective To establish a solid-phase extraction method for the simultaneous extraction of 39 kinds of poisons in urine and analyze them by high performance liquid chromatography. Methods Doxapram as an internal standard, 1ml urine sample by Oasis solid phase extraction, analysis by HPLC. Results 39 kinds of drug toxins can be put forward simultaneously from the urine, endogenous substances do not interfere with the determination. The absolute recovery was greater than 70% except morphine; the intra- and inter-day precision was less than 10%; the detection limit was 1-15ng / ml; the linear correlation coefficient was above 0.9977. Conclusion This method is rapid, simple, reproducible, and has little interference. It can be used in the screening of drug toxins in practical cases.