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建立超高效液相色谱法测定乳制品中纳他霉素含量的方法。方法:使用超高效液相分离系统,超声波萃取法,选用Waters BEH C18分析柱(2.1 mm×50 mm×1.7μm),甲醇-水进行梯度洗脱,紫外检测器检测,波长为305 nm,峰面积外标法定量。纳他霉素在0.05~4.00 mg/L浓度范围内,其浓度和峰面积有良好的线性关系,相关系数R=0.999 95。针对液态乳样品加标,平均回收率在93.7%~106.4%之间,RSD≤6.49%(n=6)。此方法前处理简单,准确、重现性好,且最低检出限(液态乳0.10 mg/L,乳粉0.30 mg/kg)低于国标方法,具有较大优势。此方法适用于乳及乳制品中纳他霉素的测定。
To establish a method for the determination of natamycin in dairy products by ultra performance liquid chromatography. Methods: Ultra-high performance liquid chromatography (HPLC) and ultrasonication were used. Waters BEH C18 column (2.1 mm × 50 mm × 1.7 μm), gradient elution with methanol-water and UV detector were used. Area of external standard method of quantitative. Natamycin in the concentration range of 0.05 ~ 4.00 mg / L, the concentration and peak area has a good linear relationship, the correlation coefficient R = 0.99995. For spiked liquid milk samples, the average recoveries ranged from 93.7% to 106.4% with a RSD ≤ 6.49% (n = 6). The pretreatment method is simple, accurate and reproducible. The lowest detection limit (liquid milk 0.10 mg / L, milk powder 0.30 mg / kg) is lower than the national standard method, which has great advantages. This method is suitable for the determination of natamycin in milk and dairy products.