实验研究吸入相当车间空气中最高容许浓度(ПАК)1000mg/m~3乙醇观察孕鼠血、羊水及胎血中乙醇含量的变化;分析乙醇敏感性不同的孕鼠对胎鼠发育的关系。选用性成熟大鼠,于实验前2～3周按每克体重3.5g剂量经腹腔注射25%乙醇溶液,根据睡眠时间长短分为:高、中和低乙醇耐受组。各组间大鼠交配妊娠后,将孕鼠移置染毒柜内作1000mg/m~3乙醇浓度吸入染毒,每天4h,每周5d。对照组吸入清洁空气。每隔5d取血用气相色谱仪测定孕鼠血中乙醇含量。妊娠第20天断头处死动物,取羊水和胎血测乙醇并测量胎儿体重和头尾长。全部实验共用150只性成熟大鼠和1087只妊娠第20天胎仔。按乙醇耐受性分组,进行组间和实验组与对照组间比较。 Experimental study inhalation of the workshop air of the highest allowable concentration (ПАК) 1000mg / m ~ 3 ethanol observed pregnant rats blood, amniotic fluid and fetal blood alcohol content changes; analysis of ethanol sensitivity of pregnant rats on fetal development. Sexually mature rats were randomly selected and injected intraperitoneally with 25% ethanol at a dose of 3.5g per gram of body weight 2 to 3 weeks before the experiment. According to the length of sleep, they were divided into high, medium and low alcohol tolerance groups. Rats in each group after mating pregnancy, the pregnant rats transplanted into the poisoning cabinet 1000mg / m ~ 3 ethanol concentration inhalation, daily 4h, 5d week. Control group inhaled clean air. Blood was collected every 5 days using a gas chromatograph to determine the level of ethanol in pregnant rats. On the 20th day of gestation, the animals were decapitated, the amniotic fluid and fetal blood were taken to measure the ethanol, and the fetal weight and head-tail length were measured. All the experiments shared 150 sexual maturity rats and 1087 pregnancies on the 20th day fetus. By ethanol tolerance group, the comparison between groups and experimental group and control group.