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目的:本研究试图证明湖北产牛至不同部位所得精油存在化学成分和抗氧化性的差异,并为开发牛至药材新的药用部位提供实验依据及参考。创新点:首次对湖北产牛至的“地上部分”和“地下部分”进行了化学成分和抗氧化活性的对比研究,结果表明“地下部分”同样是有效的药用部位。方法:采用气相色谱-质谱联用法(GC-MS)对牛至不同部位(花-叶、茎、根)所得精油的化学成分进行比较与分析(附表1),同时结合二苯三硝基苯肼(DPPH)自由基清除率实验和铁氰化钾总还原能力实验考察了各部位精油的抗氧化性(图1、图2和表1),为牛至精油抗氧化活性的研究进行了新的尝试。结论:湖北产牛至药材的“地下部分”(根)与“地上部分”(花-叶)的精油具有类似的抗氧化作用,具有一定的药用价值。牛至精油在作为抗氧剂使用时,“地下部分”(根)不应被忽略,而应适当保留或开发。
OBJECTIVE: This study attempts to demonstrate the difference in chemical composition and antioxidation of essential oil from different parts of oregano produced in Hubei Province, and provide experimental basis and references for the development of new medicinal parts of oregano medicinal materials. Innovative point: For the first time, the chemical composition and antioxidant activity of “underground part ” and “underground part ” of oregano in Hubei Province were compared. The results showed that “underground part ” is also a valid medicinal part . Methods: The chemical constituents of essential oils from different parts of oregano (flower-leaf, stem, root) were compared and analyzed by GC-MS (Table 1), and the combination of DBT The experiments of free radical scavenging of phenylhydrazine (DPPH) and the total reducing ability of potassium ferricyanide investigated the antioxidant activity of essential oils in different parts (Fig. 1, Fig. 2 and Table 1), and studied the antioxidant activity of oregano essential oil New attempt. Conclusion: The essential oil of “underground part ” (root) and “aerial part ” (flower - leaf) of Oregano medicinal in Hubei has similar antioxidant effect and has certain medicinal value. Oregano Essential Oil When used as an antioxidant, “underground parts” (roots) should not be ignored but should be properly retained or developed.