目的:探讨脉冲高强度聚焦超声局部辐照荷瘤裸鼠后,对经尾静脉输入的报告基因在瘤体中转染和表达的影响。材料与方法:于雌性C3H裸鼠(n=3)两侧腋窝皮下接种鳞癌组织块,待肿瘤长到体积约0.4cm3时进行实验。每只裸鼠一侧肿瘤采用脉冲高强度聚集超声辐照,另一侧作为对照。构建报告基 OBJECTIVE: To investigate the effect of high-intensity focused ultrasound on the transfection and expression of the reporter gene introduced into the tail vein of tumor-bearing nude mice. MATERIALS AND METHODS: Squamous cell carcinomas were inoculated subcutaneously in the armpit of female C3H nude mice (n = 3), and the experiment was performed when the tumor grew to a volume of about 0.4cm3. The tumor on one side of each nude mouse was irradiated by pulsed high-intensity ultrasound and the other side served as a control. Build a report base