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从垂死病鳖的肝脏分离到９６－１２－Ⅳ菌株，用该菌株人工感染健康鳖，感染率１００％。由感染发病鳖的肝脏分离到９６－１２－Ⅳ’菌株，经生理生化反应测定，与９６－１２－Ⅳ菌株的测定结果一致，鉴定为脑膜炎败血性黄杆菌。该菌通过血道传播，侵袭全身各脏器，使各脏器组织发生变质性病变，淤血、出血，呈败血症。选择１４种药物以纸片扩散法对该菌进行药敏试验，证实其中４种药物抑菌作用较强，用它们配制了鳖必康Ⅲ号，临床验证效果良好。
The 96-12-Ⅳ strain was isolated from the liver of the moribund disease turtle and infected with healthy turtle with this strain. The infection rate was 100%. The 96-12-IV ’strain was isolated from the liver of infected turtle and identified by physiological and biochemical reaction, which was consistent with the result of 96-12-Ⅳ strain. It was identified as meningococcus flavum. The bacteria spread through the bloodstream, the invasion of various organs of the body so that the deterioration of organ tissue lesions, congestion, bleeding, was sepsis. Fourteen drugs were selected to conduct drug susceptibility tests on the bacteria by disk diffusion method. It was confirmed that four of the four drugs showed strong antibacterial activity. They were formulated with tuibikang Ⅲ, and the clinical validation results were good.