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目的:研究NFkB1基因启动子-94ins/del ATTG与中国汉族人溃疡性结肠炎的相关性.方法:采用PCR限制性片段长度多态性分析(PCR-RFLP)法,检测73例中国湖北汉族溃疡性结肠炎患者与121例健康对照者NFkB1基因启动子-94ins/del ATTG基因型,分析该基因多态性与溃疡性结肠炎以及临床亚型的相关性,并将该结果与已报道的意大利人群中该位点多态性分布进行比较.结果:在NFkB1基因启动子-94ins/del ATTG位点UC组的基因型频率、等位基因频率、临床亚型与正常对照组比较无显著相关性(P>0.05);然而,中国湖北汉族人群与意大利高加索人群在该位点的基因型(χ2=13.155,P<0.05)和等位基因(OR=0.566,95%CI0.413-0.774,P<0.01)频率分布有显著差异.结论:NFkB1基因启动子区域多态性与中国湖北汉族人溃疡性结肠炎无显著相关性.
OBJECTIVE: To investigate the association between NF-κB1 promoter-94ins / del ATTG and Chinese Han population with ulcerative colitis.METHODS: PCR-RFLP method was used to detect 73 Han Chinese patients with ulcer The NF-κB gene promoter-94ins / del ATTG genotypes in patients with colitis and 121 healthy controls were analyzed. The association of the polymorphism with ulcerative colitis and clinical subtypes was analyzed. The results were compared with those reported in Italy The distribution of polymorphism in this locus was compared.Results: There was no significant correlation between genotype frequency, allele frequency and clinical subtype in UC group of NF-κB gene promoter -94ins / del ATTG (P> 0.05). However, genotypes at this locus (χ2 = 13.155, P <0.05) and alleles (OR = 0.566, 95% CI0.413-0.774, P <0.01) .Conclusion: There is no significant correlation between NFkB1 promoter region polymorphism and ulcerative colitis in Chinese Han population of Hubei province.