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首先以逆转录－ＤＮＡ聚合酶链式反应（ＲＴ－ＰＣＲ）从人肺成纤维细胞中钓取全长成纤维细胞生长因子受体１（ＦＧＦＲ１）和成纤维细胞生长因子受体１（ＦＧＦＲ１）ｃＤＮＡ，然后分别克隆入真核细胞表达载体ｐＲＣ／ＣＭＶ质粒中，将重组质粒ｐＲＣ／ＣＭＶ－ＦＧＦＲ１和ｐＲＣ／ＣＭＶ－ＦＧＦＲ１分别及共转染小鼠肌母细胞Ｌ６中，经３Ｈ－ＴｄＲ掺入试验结果表明ＦＧＦＲ１对细胞增生起负调节作用
FGFR1 and fibroblast growth factor receptor 1 (FGFR1) were first obtained from human lung fibroblasts by reverse transcription-polymerase chain reaction (RT-PCR) The recombinant plasmids pRC / CMV-FGFR1 and pRC / CMV-FGFR1 were cotransfected into mouse myoblast L6 respectively, and the recombinant plasmids pRC / CMV-FGFR1 and pRC / CMV-FGFR1 were cotransfected into the eukaryotic cell expression vector pRC / TdR incorporation test results show that FGFR1 起 play a negative regulatory effect on cell proliferation