放射免疫测定技术已经广泛应用于测定激素、蛋白质、前列腺素、药物、胆酸、环核苷酸、神经介质等,但应用于酶测定的报告尚少。1976年 Roberts 等应用于测定 CPK同功酶 MM 获得成功,此后相继报告CPK-BB,MB 的测定,一些作者认为用放免技术测定酶比化学方法优越,敏感性强,特异性高,同时能反映有活性和无活性的酶成份的改变,是唯一的直接测定酶蛋白的方法,因而是一个新的领域。为了探索放 Radioimmunoassay has been widely used in the determination of hormones, proteins, prostaglandins, drugs, cholic acid, cyclic nucleotides, neurotransmitters, etc. However, there are few reports on enzyme assays. 1976 Roberts and other applications in the determination of CPK isozyme MM was successful, since then have reported CPK-BB, MB determination, some authors believe that radioimmunoassay using enzyme technology than the superior, sensitive, high specificity, and can reflect The change of the active and inactive enzyme components is the only method that directly measures the enzyme protein and is therefore a new field. In order to explore put