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目的:通过检测髓母细胞瘤(Medulloblastoma)中凋亡抑制蛋白(survivin)的表达情况,探讨它的临床意义,为髓母细胞瘤的治疗寻求新的思路。方法:对50例髓母细胞瘤及10例正常脑组织石蜡切片行免疫组化检测,通过对survivin蛋白在髓母细胞瘤患者与正常脑组织中的表达差异,并运用相关统计分析方法分析它在不同性别、年龄段、肿瘤大小、病理类型、临床分期的表达差异及其与预后的相关性。结果:(1)髓母细胞瘤中survivin蛋白阳性表达率为72%,明显高于正常脑组织的阳性表达率0%,二者表达差异明显(P<0.01);亚组分析发现在survivin蛋白阳性组(n=36)和阴性组(n=14)病例中,性别分布、肿瘤部位、肿瘤大小、病理分型等因素在两者间无明显差别(P>0.05),而年龄及TM临床分期在两组间差异显著(P分别为0.044和0.027)。(2)survivin阳性患者预后与和阴性组相比差异显著(P=0.001)。结论:survivin可能参与了对髓母细胞瘤细胞凋亡的调控,并对髓母细胞瘤的发生发展可能起着重要作用。由于survivin蛋白表达与髓母细胞瘤患者年龄、临床分期及预后等也密切相关,可作为判断病情和评价预后的指标。
Objective: To explore the clinical significance of survivin expression in Medulloblastoma and seek new ideas for the treatment of medulloblastoma. Methods: Fifty cases of medulloblastoma and 10 cases of normal brain tissue paraffin sections were examined by immunohistochemistry. The expression of survivin protein in medulloblastoma patients and normal brain tissues was analyzed by using statistical analysis The difference of the expression in different sex, age, tumor size, pathological type, clinical stage and its correlation with prognosis. Results: (1) The positive rate of survivin protein in medulloblastoma was 72%, which was significantly higher than that in normal brain tissue (0%) (P <0.01). The subgroup analysis showed that survivin protein In the positive (n = 36) and negative (n = 14) cases, there were no significant differences in gender distribution, tumor location, tumor size and pathological type between the two groups (P> 0.05) Staging differed significantly between the two groups (P = 0.044 and 0.027, respectively). (2) The prognosis of survivin-positive patients was significantly different from that of the negative group (P = 0.001). Conclusion: Survivin may be involved in the regulation of apoptosis in medulloblastoma cells and may play an important role in the development of medulloblastoma. Because survivin protein expression and age, clinical stage and prognosis of patients with medulloblastoma are also closely related, can be used as indicators to determine the condition and evaluate the prognosis.