Relationships between carbon (C) production and nitrogen (N) mineralization were investigated in two alpine wetland soils of the Tibetan Plateau using laboratory incubation under different temperatures (5, 15, 25, and 35 ℃) and water saturation (noninundation and inundation). A significant positive relationship was found between CO2 production and N mineralization under increasing temperatures from 5 to 35 ℃ with the same water saturation condition in the marsh soil (r2 ＞ 0.49, P ＜ 0.0001) and the peat soil (r2 ＞ 0.38, P ＜ 0.002), and a negative relationship with water saturation increasing at the same temperature, especially 25 and 35 ℃, in the marsh soil (r2 ＞ 0.70, P ＜ 0.009) and the peat soil (r2 ＞ 0.61, P ＜ 0.013). In conclusion, temperatures and water saturation could regulate the relationship between CO2 production and net N mineralization in the Tibetan alpine marsh and peat soils.