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目的:通过对比观察米非司酮对早孕蜕膜组织中白血病抑制因子(LIF)表达的影响,探讨米非司酮终止早孕的分子免疫机制。方法:采用免疫组化染色测定蜕膜组织中LIF的表达,其中正常早孕人工流产组20例,米非司酮药物流产组22例。同时采用放射免疫法测定两组孕妇血清孕酮及人绒毛膜促性腺激素(HCG)水平。结果:LIF在正常早孕蜕膜组织中均呈阳性表达,主要在蜕膜组织腺体和内膜细胞染色较明显。LIF在米非司酮药物流产组表达强度较人工流产组明显降低(P<0.01);药物流产组血清孕酮及HCG水平显著低于人工流产组(P<0.01)。结论:米非司酮明显降低LIF在蜕膜组织中的表达,引起HCG及孕酮分泌下降,从而影响胚胎发育引起流产,达到终止早孕的目的。
OBJECTIVE: To compare the effect of mifepristone on the expression of leukemia inhibitory factor (LIF) in decidua in early pregnancy and to explore the molecular mechanism of mifepristone termination of early pregnancy. Methods: The expression of LIF in decidua tissue was determined by immunohistochemical staining, including 20 cases of abortion in normal early pregnancy and 22 cases of mifepristone abortion. Serum progesterone and human chorionic gonadotropin (HCG) levels were measured by radioimmunoassay in both groups. Results: LIF in normal early pregnancy decidua were positive, mainly in the decidual tissue gland and endometrial cells staining more obvious. The expression intensity of LIF in the mifepristone abortion group was significantly lower than that of the induced abortion group (P <0.01). The serum progesterone and HCG levels in the abortion group were significantly lower than those of the induced abortion group (P <0.01). Conclusion: Mifepristone significantly reduces the expression of LIF in decidual tissue, causing a decrease in the secretion of HCG and progesterone, thereby affecting embryo development and causing abortion, thus ending the purpose of early pregnancy.