体外培养皮肤及口腔粘膜对研究口腔及皮肤疾病毒理学及致癌机理具有重要意义。该方法无需使用大量活体动物,较之动物模型有很多优越性,且已被用于研究不同部位的口腔上皮细胞角蛋白表达。为获得正常的复层鳞状上皮,早期用去表皮的带有完整基底膜的人类真皮,暴露在空气中培养,最近使用胶原/成纤维细胞基质,气/液面培养,使之在基质上形成复层上 In vitro culture of the skin and oral mucosa on oral and dermal disease toxicology and carcinogenesis is of great significance. This method does not require the use of large numbers of living animals and has many advantages over animal models and has been used to study oral epithelial keratin expression at different sites. In order to obtain normal stratified squamous epithelium, an early epidermis of human dermis with an intact basement membrane is exposed to air for culture, most recently using a collagen / fibroblast matrix, air / liquid culture to make it on the substrate Formation of the complex on