论文部分内容阅读
目的观察高胆红素血症致脑瘫动物模型黑质纹状体系多巴胺能神经元数量及超微结构的变化,为深入研究手足徐动型脑瘫的神经病理学机制奠定基础,也为临床用药和早期康复提供依据。方法生后2～5日龄仔兔30只,随机分为对照组10只和模型组20只。模型组给予腹腔分次注射胆红素,总量为300mg/kg体重;对照组给予腹腔注射同等剂量的生理盐水。注射后2组仔兔均母乳喂养至45d。将鉴定为脑瘫模型的仔兔和对照组仔兔均处死,TH染色进行神经元定量分析及超微结构观察。结果①模型组黑质及纹状体神经元超微结构较对照组有明显改变;②模型组黑质及纹状体TH阳性神经元数明显少于对照组(P<0.01)。结论脑瘫仔兔黑质及纹状体超微结构明显改变,黑质纹状体系多巴胺能神经元损伤,神经元数量减少。
Objective To observe the changes of the number and ultrastructure of dopaminergic neurons in substantia nigra and striatum induced by hyperbilirubinemia in animal models of cerebral palsy, and lay a foundation for further study on the neuropathological mechanism of hand-foot-beating cerebral palsy. Rehabilitation provide the basis. Methods Thirty rabbits aged 2 ~ 5 days were randomly divided into control group (n = 10) and model group (n = 20). The model group was given intraperitoneal injection of bilirubin, the total amount of 300mg / kg body weight; control group was injected intraperitoneally with the same dose of saline. Two groups of rabbits were breast-fed to 45 days after injection. The rabbits in the model of cerebral palsy were sacrificed and the rabbits in the control group were sacrificed. TH staining was used for neuron quantitative analysis and ultrastructure observation. Results ① The ultrastructure of substantia nigra and striatum neurons in the model group was significantly changed compared with the control group. ② The number of TH positive neurons in substantia nigra and striatum of the model group was significantly less than that of the control group (P <0.01). Conclusions The ultrastructure of substantia nigra and striatum in rabbits with cerebral palsy obviously changes. The dopaminergic neurons in nigrostriatal system are damaged and the number of neurons is decreased.