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目的分析右美托咪定对脓毒症大鼠炎性递质的影响。方法选取雄性大鼠60只,随机分成3组,分别为正常组、脓毒症组以及治疗组,每组20只。正常组大鼠静注氯化钠溶液,脓毒症组和治疗组借助静脉注射毒素建立脓毒症大鼠模型,但采取不同的治疗方法,定时抽血,对脓毒症大鼠模型48 h以内活动变化和病死率进行对比。采取ELISA法对大鼠血液的初期炎性递质肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)以及晚期大鼠炎性递质高迁移率族蛋白B1(HMGB1)的含量改变情况进行检测。结果在TNF-α、IL-6、HMGBl含量变化中,脓毒症组与治疗组均呈先上升后下降,正常组相对平稳,与正常组相比,其他2组含量均增高,差异存在统计学意义(P<0.05)。治疗组与脓毒症组比较,各指标上升趋势相近,下降过程治疗组更明显,且治疗组各指标含量比脓毒症组低,差异显著(P<0.05)。3组病死率比较,脓毒症组>治疗组>正常组,差异存在统计学意义(P<0.05)。结论右美托咪定能降低脓毒症大鼠的炎症递质表达,从而降低脓毒症大鼠的病死率。
Objective To analyze the effect of dexmedetomidine on inflammatory mediators in septic rats. Methods Sixty male rats were randomly divided into three groups: normal group, sepsis group and treatment group, with 20 rats in each group. Rats in normal group were injected intravenously with sodium chloride solution, sepsis group and treatment group. The sepsis model was established by intravenous injection of toxins. Different treatment methods were used to take blood regularly. Changes in activity and mortality compared. The serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and inflammatory mediators HMGB1 (HMGB1) ) Changes in the content of the test. Results In the changes of TNF-α, IL-6 and HMGB1 contents, the sepsis group and the treatment group first increased and then decreased, the normal group was relatively stable, the content of the other two groups increased compared with the normal group, the difference was statistically Significance (P <0.05). Compared with the sepsis group, the rising trend of each index was similar in the treatment group and the decreasing process group was more obvious, and the content of each index in the treatment group was lower than that in the sepsis group (P <0.05). Three groups of mortality, sepsis group> treatment group> normal group, the difference was statistically significant (P <0.05). Conclusion Dexmedetomidine can reduce the expression of inflammatory mediators in septic rats and thus reduce the mortality of septic rats.