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根据沃森-克里克碱基互补配对原则,利用DNA模板将反应物进行选择性组合,通过DNA模板法成功合成了不对称席夫碱金属配合物[Zn2Len Cl2](L=D4-4H-D5-2,2、D4=端位修饰氨基的单链DNA4、D5=端位修饰醛基的单链DNA5、D4和D5互补配对,4H=2,6-二甲基苯酚、2,2=(R)-(+)-1,1’-联-2-萘胺、en=乙二胺),通过超滤及非变性凝胶电泳对产物进行分离提纯,并用紫外-可见分光光谱对其进行表征。荧光性质研究表明,在室温条件下,不对称席夫碱配合物中电子发生了π*→π、π*→n跃迁,在大约470 nm处出现强的荧光发射峰。
According to Watson-Crick base-pairing principle, DNA templates were used to selectively synthesize the reactants. The asymmetric Schiff base metal complex [Zn2Len Cl2] (L = D4-4H- D5-2,2, D4 = single-stranded DNA with amino group at terminal modification 4, D5 = single-stranded DNA with terminal modification aldehyde group 5, complementary pairing of D4 and D5, 4H = 2,6-dimethylphenol, (R) - (+) - 1,1’-bi-2-naphthylamine, en = ethylenediamine). The products were isolated and purified by ultrafiltration and non-denaturing gel electrophoresis. The products were characterized by UV-visible spectroscopy Characterization. Fluorescence properties showed that π * → π and π * → n transitions occurred in the asymmetric Schiff base complexes at room temperature, and strong fluorescence emission peak appeared at about 470 nm.