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目的研究结直肠癌组织中血管内皮生长因子(VEGF)和血管形成因子-2(angiopoietin-2)及纤连蛋白(FN)的表达情况及其与结直肠癌肝转移的关系。方法获取结直肠癌标本60例,分别用SQRT-PCR和免疫组织化学染色法检测VEGF、血管形成因子-2、FN的mRNA转录和蛋白表达水平。结果结直肠癌组织中VEGF、血管形成因子-2和FN的mRNA转录和蛋白表达水平增高(P<0.05)。VEGF表达水平与肿瘤浸润深度、淋巴结和/或肝转移、Dukes分期有关(P<0.05)。血管形成因子-2则仅与癌组织的分化程度有关(P<0.05)。FN在细胞外基质(ECM)中的表达高于癌旁组织(P<0.05);而在基底膜中的表达低于癌旁组织(P<0.05);两者均与癌浸润深度和淋巴结转移有关(P<0.05)。而基底膜中FN的缺失同时与肝转移有关(P<0.05)。结论结直肠癌细胞产生的VEGF可能通过促进肿瘤新生血管的生成而促进血行转移和转移灶的形成,血管形成因子-2与VEGF共同促进肿瘤血管的生成,而FN在肿瘤组织基底膜中的缺失是肝转移的重要起始因素。
Objective To investigate the expression of vascular endothelial growth factor (VEGF), angiopoietin-2 and fibronectin (FN) in colorectal cancer and its relationship with hepatic metastasis of colorectal cancer. Methods Sixty colorectal cancer specimens were obtained. The mRNA and protein expressions of VEGF, angiogenic factor-2 and fibronectin were detected by SQRT-PCR and immunohistochemical staining respectively. Results The mRNA and protein expressions of VEGF, angiogenic factor-2 and fibronectin in colorectal cancer tissues were increased (P <0.05). The expression of VEGF correlated with the depth of tumor invasion, lymph node and / or liver metastasis and Dukes stage (P <0.05). Angiogenic factor-2 was only associated with the degree of differentiation of cancer (P <0.05). The expression of FN in extracellular matrix (ECM) was higher than that in paracancerous tissues (P <0.05), while it was lower in paracancerous tissues than in paracancerous tissues (P <0.05). Both of them were correlated with the depth of invasion and lymph node metastasis Related (P <0.05). However, the loss of FN in the basilar membrane correlated with liver metastasis (P <0.05). Conclusion The VEGF production in colorectal cancer cells may promote the formation of hematogenous metastasis and metastasis through the promotion of neovascularization. Together with VEGF, angiogenic factor-2 promotes the formation of tumor blood vessels, while the deletion of FN in the basement membrane of tumor tissue Liver metastasis is an important starting factor.