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目的:研究许旺细胞(Schwann Cell,SC)能否促进离体多巴胺(Dopamine,DA)神经元存活和生长。方法:取生后2~3d Sprague Dawley(SD)大鼠周围神经,培养获得SC,换以N-2培养液培养2d,收集作为SC培养液。取胎龄14d SD大鼠中脑腹侧细胞;用SC培养液培养该细胞,对照组用N-2培养液培养。用酪氨酸羟化酶(Tyrosine hydroxylase,TH)免疫细胞化学方法,观察DA神经元生长情况。结果:实验组TH(+)细胞明显多于对照组,且突起多而长。统计分析两者差异有显著意义。结论:SC培养液能促进离体DA神经元存活和生长。
Objective: To investigate whether Schwann cell (SC) can promote the survival and growth of dopaminergic neurons in vitro. METHODS: Peripheral nerves of Sprague Dawley (SD) rats were collected 2 ~ 3d days after their birth. SCs were obtained after culture for 2 days and were collected as SC culture medium. The ventral cells of midbrain of 14-day-old SD rats were obtained. The cells were cultured in SC medium and the control group were cultured in N-2 medium. Tyrosine hydroxylase (TH) immunocytochemistry was used to observe the growth of DA neurons. Results: TH (+) cells in the experimental group were significantly more than those in the control group, with more and more protrusions. Statistical analysis of the difference between the two significant. Conclusion: SC medium can promote the survival and growth of isolated DA neurons.