Research on the development of the optical frequency standard based on trapped and cold 40 Ca+ with the 4s2S1/2-3d2D5/2 clock transition at 729nm is reported. A single calcium ion was trapped and laser cooled in the Paul trap and stay in trap for more than 15 days. The linewidth of a 729nm laser was reduced to less than 10Hz by locking to a cavity for longer than 50 hours uninterruptedly. The overall systematic uncertainty of the clock transition has been characterized to be better than 6.5×10 16 . The absolute frequency of the clock transition was measured at 10 15 level using an optical frequency comb referenced to a Hydrogen maser, which was calibrated to the SI second through the global positioning system (GPS). The frequency value was 411042129776393.0(1.6)Hz after the correction of the systematic shifts. Research on the development of the optical frequency standard based on trapped and cold 40 Ca + with the 4s2S1 / 2-3d2D5 / 2 clock transition at 729nm is reported. A single calcium ion was trapped and laser cooled in the Paul trap and stay in trap for The linewidth of a 729nm laser was reduced to less than 10Hz by locking to a cavity for longer than 50 hours uninterruptedly. The overall systematic uncertainty of the clock transition has been to be better than 6.5 × 10 16. absolute frequency of the clock transition was measured at 10 15 level using an optical frequency code referenced to a Hydrogen maser, which was calibrated to the SI second through the global positioning system (GPS). The frequency value was 411042129776393.0 (1.6) Hz after the correction of the systematic shifts.