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用间接免疫荧光方法，将神经肽Ｙ Ｙ１ 受体免疫反应物质（ＬＩ）定位于大鼠腰段背根节小神经元和脊髓Ⅱ层神经元近细胞膜处。用共聚焦显微术显示背根神经元中Ｙ１ 受体－ＬＩ呈点状分布于神经元表面。在电子显微镜下发现Ｙ１ 受体－ＬＩ主要定位于背根节小神经元细胞膜下被膜囊泡、内在小体和管状池，有时也定位于细胞膜上。在脊髓Ⅱ层中，Ｙ１ 受体－ＬＩ定位于神经元细胞膜，许多树突为Ｙ１ 受体阳性，与未被标记的轴突终末形成突触；Ｙ１ 受体－ＬＩ主要定位于突触后膜以外的细胞膜，只有在少数情况下定位在突触后膜。表明在大鼠背根节小神经元中Ｙ１ 受体为胞体受体，并参与受体内在化过程；在脊髓背角神经元中，Ｙ１ 受体是连接后／突触后受体。
The indirect immunofluorescence method was used to locate the neuropeptide Y Y1 receptor immunoreactive substance (LI) in the rat lumbar dorsal root ganglion neurons and spinal cord layer II neurons near the plasma membrane. Confocal microscopy showed that the Y1 receptor-L1 in the dorsal root neurons showed a dot-like distribution on the surface of neurons. It was found under the electron microscope that the Y1 receptor-L1 mainly located in the dorsal root ganglion neurons sub-membrane capsule vesicles, inner bodies and tubular pool, and sometimes located in the cell membrane. In the spinal cord layer, the Y1 receptor-L1 locates in the neuronal cell membrane. Many dendrites are positive for the Y1 receptor and form synapses with unlabeled axonal terminals. The Y1 receptor-L1 is mainly located in the postsynaptic Membrane membranes other than the membrane, only in a few cases located in the postsynaptic membrane. It is suggested that Y1 receptor is a cell body receptor in rat dorsal root ganglion neurons and participates in the process of receptor internalization. In spinal dorsal horn neurons, Y1 receptor is a post-junction / postsynaptic receptor.