Objective:To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivationreperfusion(OGD/RO)-induced injury in SH-SY5 Y cells.Methods:SH-SY5 Y cells were divided into a control group,a OGD/RO group,which was subject to OGD/RO induction;and 3 baicalin groups subject to baicalin(1,5,25 μ mol/L) for 2 h before induction of OGD/RO(low-,medium-,and high-dose baicalin groups).Cell viability was detected by thiazolyl blue tetrazolium bromide(MTT) assay and flow cytometric analysis was used to detect cell apoptosis.Real-time polymerase chain reaction was performed to determine the mRNA expression of caspase-3gene.Western blot analysis was conducted to determine the expression of nuclear factor(NF)- k B and N-methyl-daspartic acid receptor-1(NMDAR1).Results:Baicalin could significantly attenuate OGD/RO mediated apoptotic cell death in SH-SY5 Y cells;the apoptosis rates in the low-,medium- and high-dose groups were 12.1%,7.9%,and 5.4%,respectively.Western blot and real-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD/RO group(P<0.01).Additionally,down-regulation of NF-κB and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD/RO group.Compared with the low-dose baicalin group,remarkable decrease was noted in the medium- and high-dose groups(P<0.01).Conclusion:Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis. Objective: To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivationperfusion (OGD / RO) -induced injury in SH-SY5 Y cells. Methods: SH-SY5 Y cells were divided into a control group, a OGD / RO group, which was subject to OGD / RO induction; and 3 baicalin groups subject to baicalin (1,5,25 μ mol / L) for 2 h before induction of OGD / RO (low-, medium-, and high-dose baicalin groups). Cell viability was detected by thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometric analysis was used to detect cell apoptosis. Real-time polymerase chain reaction was performed to determine the mRNA expression of caspase-3 gene. Western blot analysis was conducted to determine the Expression of nuclear factor (NF) -k B and N-methyl-daspartic acid receptor-1 (NMDAR1). Results: Baicalin could be attenuated by OGD / RO- mediated apoptotic cell death in SH- SY5 Y cells; the apoptosis rates in the low -, medium- and high-dose groups were 12.1%, 7.9%, and 5.4% respectively. Western blot and rea l-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD / RO group (P <0.01) .Additionally, down-regulation of NF-κB and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD / RO group. Compared with the low-dose baicalin group, remarkable decrease was noted in the medium- and high-dose groups (P <0.01) .Conclusion: Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis.