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目的:观察载体蛋白偶联的TSO45-4B抗原FnⅢ结构域相应的线性B细胞表位肽诱导的体液免疫反应。方法:人工合成TSO45-4B抗原FnⅢ结构域2条预测表位肽,偶联钥孔血蓝蛋白免疫小鼠,采用ELISA法检测小鼠血清中预测表位肽特异性抗体滴度。结果:免疫小鼠血清中检测到1条预测表位肽特异性抗体,其效价达到1∶1 280。结论:设计的1条TSO45-4B抗原FnⅢ结构域线性B细胞表位肽可诱导小鼠产生体液免疫反应。
OBJECTIVE: To observe the humoral immune response induced by the corresponding linear B-cell epitope peptide of FnⅢ domain of carrier protein-coupled TSO45-4B antigen. METHODS: Two predicted epitope peptides of FnⅢ domain of TSO45-4B antigen were synthesized. Mice were immunized with keyhole limpet hemocyanin and the titer of epitope peptide specific antibody in serum was detected by ELISA. Results: One epitope-specific peptide-specific antibody was detected in the serum of immunized mice and its titer reached 1: 280. CONCLUSION: The designed Bn epitope of FnⅢ domain of TSO45-4B antigen can induce humoral immune response in mice.