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目的 检测微卫星DNA序列的不稳定性在中国人原发性食管癌中的表达并探讨其与食管癌临床病理特征之间的关系。 方法 应用聚合酶链反应-聚丙烯酰胺凝胶电泳-硝酸银染色技术,检测63例中国人原发性食管癌组织中微卫星DNA序列的不稳定性。 结果 食管癌中微卫星DNA序列不稳定性的发生率为41.2%;微卫星DNA序列的不稳定性与肿瘤的病理类型有关(P<0.05),而与临床分期、病理分级、淋巴结转移、癌组织的侵袭性等无关。结论 中国人食管癌组织中存在微卫星DNA序列的不稳定性,可能是食管癌发生过程中的早期事件,并且可能更多的参与了食管小细胞癌的发生
Objective To detect the expression of microsatellite DNA sequence instability in Chinese patients with primary esophageal cancer and to explore its relationship with clinicopathological features of esophageal cancer. Methods Polymerase chain reaction-polyacrylamide gel electrophoresis-silver nitrate staining was used to detect the instability of microsatellite DNA sequence in 63 Chinese primary esophageal cancer tissues. Results The incidence of microsatellite DNA instability in esophageal cancer was 41.2%. The instability of microsatellite DNA sequence was related to the pathological type (P <0.05), but not to the clinical stage, pathological grade, lymph node metastasis, Invasion and other organizations has nothing to do. Conclusion The instability of microsatellite DNA sequences in Chinese esophageal cancer tissues may be an early event in the pathogenesis of esophageal cancer and may be more involved in the occurrence of esophageal small cell carcinoma