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目的:对芎归汤有效组分抗东莨菪碱致痴呆小鼠学习记忆能力减退的改善作用进行药效评价,并确定芎归汤有效组分对抗痴呆模型改善学习记忆药理学效应的“量-效”关系和ED50,为进一步开展药效评价提供剂量依据。方法:取昆明种小鼠进行Morris水迷宫、DT-200跳台筛选训练,将合格的小鼠80只,雌雄各半,按体重随机分为正常对照组、东莨菪碱致痴呆模型组、药效剂量1~5组和阳性药组,实验前分别给予各自治疗药物,连续给药30天后,除正常对照组外均腹腔注射东莨菪碱造模,30 min后进行Morris水迷宫实验及跳台实验,分析比较不同剂量组之间学习记忆能力差异性。结果:小鼠Morris水迷宫实验显示,芎归汤有效组分对东莨菪碱致痴呆小鼠学习记忆能力减退有明显改善作用,可以明显缩短小鼠水迷宫潜伏期,在10.92~43.68 g/kg剂量范围内均有显著性差异,且呈现一定的剂量依赖性,其ED50为4.79g/kg。小鼠跳台实验显示,芎归汤有效组分对东莨菪碱致痴呆小鼠学习记忆能力减退有明显改善作用,可以延长小鼠跳台潜伏期和减少跳台错误次数,在2.73~43.68 g/kg剂量范围内均有显著性差异,且呈现一定的剂量依赖性,其ED50为2.70g/kg。结论:芎归汤有效组分对东莨菪碱致小鼠学习记忆能力减退有明显的改善作用,并且随着剂量的增加药效越来越明显,呈现一定的剂量依赖性,具有明显的“量-效”关系。
OBJECTIVE: To evaluate the effects of Xiongui Tang active ingredients against scopolamine-induced dementia in mice with dementia, and to determine the effective dose of Xiongui Tang against dementia model to improve the learning and memory pharmacological effects. “Relationship and ED50, to provide a dose basis for further evaluation of efficacy. Methods: Kunming mice were screened by Morris water maze and DT-200 platform. 80 qualified mice were randomly divided into normal control group and scopolamine-induced dementia model group according to body weight. ~ 5 groups and the positive drug group, respectively, before the experiment were given their own medication, continuous administration of 30 days, except for the normal control group were injected scopolamine intraperitoneal modeling, Morris water maze test and platform test after 30 min, analysis of different doses Differences between groups in learning and memory ability. Results: Morris water maze test showed that the effective components of Xiongui Tang significantly reduced the learning and memory abilities of scopolamine-induced dementia mice, and significantly shortened the latency of water maze in mice, with a range of 10.92 ~ 43.68 g / kg Significantly different, and showed a certain dose-dependent, the ED50 was 4.79g / kg. The experiment of jumping in mice showed that the effective components of Xiongui Decoction could significantly improve the learning and memory abilities of scopolamine-induced dementia mice, which could prolong the jumping latency and reduce the number of jumping trips in mice, both within the dose range of 2.73 ~ 43.68 g / kg There was a significant difference, and showed a certain dose-dependent, the ED50 was 2.70g / kg. Conclusion: The effective component of Xionggui Tang can significantly improve the learning and memory abilities of mice induced by scopolamine, and its efficacy becomes more and more obvious with the increase of dose, showing a certain dose-dependent, with obvious ” Effect "relationship.