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目的探讨反式脂肪酸对小鼠大脑抗氧化系统及ATP酶的影响。方法将48只健康SPF级昆明系雄性小鼠随机分为4组,分别为对照组和25、50、100 mg/kg反式脂肪酸染毒组,每组12只。采用灌胃方式进行染毒,染毒容量均为10ml/kg,每天1次,连续染毒12周。测定小鼠脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、ATP酶的活力和丙二醛(MDA)、总蛋白的含量。结果与对照组相比,各剂量反式脂肪酸组小鼠脑组织中的抗氧化酶SOD、GSH-Px、CAT活力均降低,而脂质过氧化物MDA的含量升高;Na~+K~+-ATP酶和Ca~(2+)Mg~(2+)-ATP酶活力均降低,差异均有统计学意义(P<0.05)。结论反式脂肪酸的摄入会引起小鼠脑组织抗氧化系统受损,同时,会降低脑组织中ATP酶的活力。
Objective To investigate the effects of trans fatty acids on antioxidant system and ATPase activity in mouse brain. Methods Forty-eight healthy Kunming mice were randomly divided into 4 groups: control group and trans-fatty acid group of 25, 50 and 100 mg / kg, 12 mice in each group. Gavage by way of exposure, exposure capacity are 10ml / kg, 1 day, continuous exposure to 12 weeks. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and ATPase and the activity of malondialdehyde (MDA) Total protein content. Results Compared with the control group, the activities of antioxidant enzymes SOD, GSH-Px and CAT in brain tissues of mice in each dose of trans-fatty acid group decreased and the content of lipid peroxide MDA increased; Na ~ + K ~ + -ATPase and Ca2 + -added Mg2 + -ATPase activity were all decreased, the difference was statistically significant (P <0.05). Conclusion The intake of trans-fatty acids can cause the antioxidant system of mouse brain tissue to be impaired. At the same time, it decreases the activity of ATPase in brain tissue.