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目的研究喉癌切缘组织病理与分子生物学的不同表现,方法用PCR-SSCP(聚合酶链反应-单链构象多态性)方法检测P~(53)基因突变情况。结果 25%(5/20)肿瘤组织有P~(53)基因突变,连续检测有突变的肿瘤组织标本切缘0.5～1.0cm,距肿瘤边缘0.5,0.6cm组织中也检测到了P~(53)基因突变,而相应部位病理观察未见癌细胞。结论提示P~(53)抑癌基因的突变与否和病理的变化无直接关系,从而推测传统以切除病理病变为基础的肿瘤切除范围由于未考虑到基因的变化而可能存在潜在危险:受内在或外在因素的影响,已突变的基因表达出来,导致肿瘤的复发。因而,建议肿瘤的外科治疗方案应将观察病理改变与分子水平的改变相结合。
Objective To study the histopathology and molecular biology of the margins of laryngeal cancer. Methods The mutation of P53 gene was detected by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism). Results P (53) gene mutation was detected in 25% (5/20) of the tumor tissues. The resection margin was 0.5 ~ 1.0 cm in the continuous detection of the tumor tissue, and P ~ (53) was also detected in the 0.5 and 0.6 cm of the tumor margin ) Gene mutation, and the corresponding parts of the pathological observation of cancer cells. The results suggest that there is no direct relationship between the mutation of P ~ (53) tumor suppressor gene and the pathological changes. Therefore, it is speculated that the traditional excision of pathological lesions based on the exclusion of the gene may be potentially dangerous: Or external factors, have been mutated gene expression, leading to tumor recurrence. Therefore, it is recommended that the surgical treatment of tumors should be combined with the observation of pathological changes and molecular changes.