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目的观察健脾补肾利湿方对高尿酸肾病模型大鼠的抗炎、抗氧化作用。方法 SD大鼠除空白组外,其余采用酵母干粉、腺嘌呤制作高尿酸肾病模型。造模成功后随机分为模型组、健脾补肾利湿组(简称脾肾组)、别嘌醇组。治疗8周,检测大鼠血尿酸(BUA)、肌酐(SCr)、尿素氮(BUN)浓度,采用ELISA法检测模型大鼠血清IL-6、TNF-α水平,采用黄嘌呤氧化酶法、二硫对二硝基苯甲酸(DTNB)直接显色法和可见光分光光度法检测SOD、GSH-PX、CAT酶活性,采用TBA法检测MDA含量。结果与空白组比较,模型组各指标差异均有统计学意义(P<0.05,P<0.01)。与模型组比较,脾肾组BUA、SCr、BUN、IL-6、TNF-α水平降低,GSH-PX、SOD、CAT酶活性升高,MDA含量下降,差异均有统计学意义(P<0.05)。与别嘌醇组比较,脾肾组降低大鼠BUA、SCr、BUN、IL-6、TNF-α水平,升高GSH-PX酶活性,降低MDA含量,差异有统计学意义(P<0.05);在升高SOD、CAT酶活性方面,两组差异无统计学意义(P>0.05)。结论健脾补肾利湿方具有较好的抗炎、抗氧化作用,可积极影响高尿酸肾病大鼠模型的微炎症及氧化应激状态。
Objective To observe the anti-inflammation and anti-oxidative effects of Jianpi Bushen Lishi recipe on rats with hyperuricemia and nephropathy. Methods SD rats were divided into blank group, the rest of the yeast powder, adenine made hyperuricemic nephropathy model. After the success of modeling were randomly divided into model group, spleen and kidney dampness group (spleen and kidney group), allopurinol group. After 8 weeks of treatment, serum creatinine (BUA), creatinine (SCr) and blood urea nitrogen (BUN) were measured. Serum IL-6 and TNF-α levels were measured by ELISA. Sulfur pair dinitrobenzoic acid (DTNB) direct colorimetric method and visible spectrophotometry SOD, GSH-PX, CAT activity, using the TBA method to detect MDA content. Results Compared with the blank group, there were significant differences among the indexes in the model group (P <0.05, P <0.01). Compared with the model group, the levels of BUA, SCr, BUN, IL-6 and TNF-α in spleen and kidney decreased, the activities of GSH-PX, SOD and CAT increased, while the levels of MDA decreased (P <0.05 ). Compared with allopurinol group, the levels of BUA, SCr, BUN, IL-6 and TNF-α in rats of spleen and kidney group were decreased, the activities of GSH-PX enzyme and the content of MDA were decreased (P <0.05) There was no significant difference between the two groups in increasing SOD and CAT activity (P> 0.05). Conclusion Jianpi Bushen Lishi recipe has good anti-inflammatory and anti-oxidative effects and can positively affect the micro-inflammation and oxidative stress status of rat model of hyperuricemia.