【摘 要】
:
Semen analysis is characterized by high levels of intra-and inter-laboratory variability,due to a low level of standardization,high subjectivity of the assessments,and problems with automated procedures.To improve consistency of laboratory results,quality
【机 构】
:
Fertility Laboratory,Department of Obstetrics and Gynaecology,Radboud University Medical Center,PO B
论文部分内容阅读
Semen analysis is characterized by high levels of intra-and inter-laboratory variability,due to a low level of standardization,high subjectivity of the assessments,and problems with automated procedures.To improve consistency of laboratory results,quality control and training of technicians are important requisites.The goals of this study are to evaluate the results of an external quality control(EQC)program and standardized training by ESHRE Basic Semen Analysis Courses(BSAC)on the variability in manual assessments of semen parameters.We performed retrospective analyses of(1)the interlaboratory variability in the Dutch EQC program and(2)the interobserver variability in BSACs for concentration,motility,and morphology assessments.EQC data showed that the interlaboratory coefficient of variation(CV)for concentration assessment decreased(range from 24.0%-97.5%to 12.7%-20.9%)but not for morphology and motility assessments.Concentration variability was lower if improved Neubauer hemocytometers were used.Morphology assessment showed highest CVs(up to 375.0%),with many outliers in the period of 2007-2014.During BSAC,a significant reduction of interobserver variability could be established for all parameters(P<0.05).The absence of an effect in the EQC program for motility and morphology might be explained by respectively the facts that motility assessment was introduced relatively late in the EQC program(since 2013)and that criteria for morphology assessment changed in time.BSAC results might have been influenced by the pretraining level of participants and the influence of external factors.Both EQC and training show positive effects on reducing variability.Increased willingness by laboratories to change their methods toward standards may lead to further improvements.
其他文献
研究以α-Si3N4和Al2O3为主要粘结剂,采用高温高压(HTHP)烧结制备PCBN复合材料.系统地评价了烧结助剂(Y2O3)添加对PCBN复合材料的显微结构和相关力学性能的影响.结果 表明,在HPHT烧结过程下,加入适量Y2O3的PCBN复合材料的力学性能有了明显的提高.随着烧结助剂的加入,促进了结合剂与cBN之间的界面结合,氮化硅的长径比得到提高.结果 增强了PCBN复合材料的致密性,使复合材料的抗弯强度和硬度增大.1700℃下含有4 wt%Y2O3复合材料相对密度为99.3%,抗弯强度为870.5
采用传统固相法成功制备了不同SiO2掺杂量的ZnO-Bi2O3基压敏陶瓷.研究了SiO2添加剂对ZnO-Bi2O3基压敏陶瓷物相组成、微观形貌和电性能的影响规律及作用机理.XRD结果表明,ZnO-Bi2O3基压敏陶瓷由ZnO主晶相和尖晶石相、富Bi相和含Si相等第二相组成.当SiO2含量增加时,ZnO晶粒尺寸逐渐减小,压敏电压梯度E1mA逐渐增大,这是由于二次相的钉扎效应所致.此外,随着SiO2含量的增加,非线性系数α先增大后减小,而泄漏电流IL先减小后增大,这是由于微观结构变化影响了晶界势垒高度.当Si
To efficiently remove all recurrent lymph nodes(rLNs)and minimize complications,we developed a combination approach that consisted of 68Gallium prostate-specific membrane antigen(PSMA)ligand positron emission tomography(PET)/computed tomography(CT)and int
Male genital tract inflammation/infection and its impact on male fertility are still widely neglected in the workup of male fertility disturbances.Apart from inconsistent definitions of such inflammatory conditions which allow no adequate diagnosis and,co
内天井是居住类建筑的重要组成部分,本文结合产业园配套公寓的特点,以江门蓬江27号地块激光产业园配套楼建设工程为例,从相关指标及优缺点进行分析,为产业园配套公寓内天井设计提供参考.
Chlamydia trachomatis(CT)infection is the most prevalent sexually transmitted bacterial disease worldwide.However,unlike that in female infertility,the role of CT infection in male infertility remains controversial.The objective of this retrospective stud
为探究风化砂的工程性质,对水泥稳定风化砂进行了力学性能试验,研究了水泥掺量和养护龄期对其抗压强度、劈裂强度和抗压回弹模量的影响,并采用扫描电镜试验对水泥稳定风化砂的微观结构进行分析.结果表明,水泥稳定风化砂抗压强度与时间的关系可以用线性函数y=at+b表示,其中t为养护时间(d).水泥掺量达到3%及以上时,其7d无侧限抗压强度达到规范的要求.水泥的掺量对于劈裂强度的提升具有明显的作用.3%的水泥掺量对稳定风化砂前期抗压回弹模量的提高效果最为显著.SEM结果显示,水泥稳定风化砂结构更为致密,颗粒之间存在着大
采用固相法制备(1-x) N0.5Bi4.5Ti4O15-xBaTiO3 (NBT-BT,x=0,0.05,0.10,0.20,0.30,0.40)高温铋层状压电陶瓷材料.用X射线衍射(XRD)、SEM扫描电镜(SEM),介电和阻抗温谱等测试方法研究了BaTiO3掺杂量对Na0.5Bi4.5Ti4O15陶瓷晶体结构和电性能的影响;结果表明:当x≤0.20时,NBT--BT陶瓷材料为单一的铋层状结构相,居里温度Tc逐渐下降;随着BaTiO3掺杂量的增加,压电常数逐渐增大后逐渐减小,介电损耗变化趋势与之相反,
The aim of this article is to assess the outcomes of a low-intensity extracorporeal shock wave therapy(LiESWT)protocol for the treatment of Peyronie\'s disease(PD).Patients treated for PD were prospectively recorded,and data were retrospectively reviewe
The purpose of our study is to investigate the prognostic value of phosphatase and tensin homolog on chromosome 10(PTEN)expression in patients with de novo metastatic castration na?ve prostate cancer(mCNPC).A total of 205 patients with mCNPC at Fudan Univ