目的 :探讨血清P5 3蛋白作为职业性肺癌早期监测指标的可能性和应用价值。方法 :对长期接触多环芳烃混合物 (PAHs)的 2 3名焦炉工和 18名沥青工进行外周血淋巴细胞染色体畸变检查初筛 ,检出染色体畸变者各16名 ,然后采用双抗夹心酶联免疫吸附试验方法 (Sandwich ELISA)测定畸变者血清P5 3蛋白水平。以 12名正常献血员为对照组。结果 :焦炉工和沥青工血清P5 3水平均值高于对照组 (P <0 .0 5 ) ,其中 10例高于对照组 ( x +2s)。结论 :血清P5 3水平可从基因水平反映PAHs对机体遗传物质的损伤作用 ,该指标有可能成为职业性肺癌早期监测的又一途径。 Objective: To explore the possibility and value of serum P53 protein as an early indicator of occupational lung cancer. Methods: Twenty-three coke oven workers and 18 asphaltene workers who were exposed to PAHs for long time were screened for chromosomal aberrations of peripheral blood lymphocytes. Chromosome aberrations were detected in 16 of them, and then double-antibody sandwich enzyme The sandwich immunoassay method (Sandwich ELISA) was used to detect the level of P5 3 protein in the aberrant. Twelve normal donors were used as control group. Results: The average serum P5 3 level of coke oven workers and asphaltene workers was higher than that of the control group (P <0.05), of which 10 cases were higher than the control group (x + 2s). CONCLUSION: Serum P53 levels can reflect the genetic damage caused by PAHs at genetic level. This index may be another way to monitor early stage of occupational lung cancer.