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目的 从细胞信号传导方面探讨长春新碱抑制胃泌素促人结肠癌细胞株ＳＷ４８０ 增殖的机理及临床意义。方法 采用噻唑氮蓝（ ＭＴＴ） 比色分析法、３Ｈ肌醇掺入法及Ｃａ２＋ 荧光测定技术和γ３２ＰＡＴＰ 掺入法，在体外观察长春新碱（ ＶＣＲ） 对５ 肽胃泌素（ＰＧ） 促人结肠癌细胞株ＳＷ４８０ 增殖的影响。结果 ＰＧ＋ ＶＣＲ 组的ＳＷ４８０ 细胞活细胞数降低，与对照组相比Ｐ＜ ０ ．０１ ，与ＰＧ 组相比 Ｐ＜ ０ ．０１ ； ＰＧ＋ ＶＣＲ 组细胞内三磷酸肌醇及Ｃａ２＋ 浓度降低，与ＰＧ 组相比 Ｐ＜０ ．０１ ，与对照组相比Ｐ＞ ０ ．０５ ； ＰＧ＋ ＶＣＲ 组膜蛋白激酶活性降低，与ＰＧ 组相比Ｐ＜ ０ ．０５ ，与对照组相比 Ｐ＞ ０ ．０５ 。结论 长春新碱可能通过肌醇脂质信号通路而影响胃泌素促人结肠癌细胞株ＳＷ４８０ 的增殖，从而为结肠癌的抗信息传导治疗提供了实验依据。
Objective To investigate the mechanism and clinical significance of vincristine inhibiting the proliferation of human colon cancer cell line SW480 by gastrin from the aspect of cell signal transduction. METHODS: The 5-peptide gastrin of vincristine (VCR) was observed in vitro using thiazolidine blue (MTT) colorimetric assay, 3H-myo-inositol incorporation assay, Ca2+ fluorescence assay, and γ-32P ATP incorporation assay. (PG) Promotes the proliferation of human colon cancer cell line SW480. Results The number of viable cells in SW480 cells in the PG+VCR group decreased, compared with the control group P< 0 . 01, compared with PG group P< 0. In the PG+ VCR group, the inositol triphosphate and Ca2+ concentration in the cells decreased, compared with the PG group P<0. 01, compared with the control group P> 0. 05; PG+ VCR group membrane protein kinase activity decreased, compared with PG group P < 0. 05, compared with the control group P> 0. 05. Conclusion Vincristine may affect the proliferation of human colon cancer cell line SW480 through gastrin lipid signaling pathway and provide experimental basis for anti-information conduction therapy of colon cancer.