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目的:探讨酪氨酸激酶抑制剂除莠霉素A(HA)对CD30+间变性大细胞淋巴瘤细胞株Karpas299和霍奇金淋巴瘤细胞株L428细胞增殖和凋亡的影响。方法:采用免疫细胞化学检测Kar-pas299和L428细胞ALK的表达,MTT法检测HA对两株细胞增殖活力的影响,流式细胞仪检测HA处理后两株细胞的细胞周期、凋亡率的变化,HE染色观察细胞凋亡改变。结果:Karpas299细胞表达ALK,L428细胞则不表达;HA对两株细胞的增殖活力均有抑制作用,呈剂量和时间依赖性,P<0.05;HA对Karpas299细胞的作用比L428更为显著,P<0.05;用HA处理24h,G1期细胞和凋亡率均高于对照组,P<0.05;Karpas299细胞高于L428细胞,P<0.05;两株细胞均出现凋亡形态改变。结论:HA明显抑制两株细胞增殖,阻滞细胞周期于G1期,以Karpas299细胞作用更为明显,其机制可能与该细胞表达酪氨酸激酶受体ALK蛋白有关。
AIM: To investigate the effects of tyrosine kinase inhibitor herbimycin A (HA) on the proliferation and apoptosis of CD30 + anaplastic large cell lymphoma cell line Karpas299 and Hodgkin’s lymphoma cell line L428. Methods: The expression of ALK in Kar-pas299 and L428 cells was detected by immunocytochemistry. The proliferation of Kar-pas299 and L428 cells was detected by MTT assay. The changes of cell cycle and apoptosis were detected by flow cytometry The changes of apoptosis were observed by HE staining. Results: Karpas299 cells expressed ALK but L428 cells did not express. HA inhibited the proliferation of both cell lines in a dose and time dependent manner (P <0.05). The effect of HA on Karpas299 cells was more pronounced than that of L428 cells and P <0.05; HA treated for 24 h, G1 phase cells and apoptosis rate were higher than the control group, P <0.05; Karpas299 cells were higher than L428 cells, P <0.05; apoptotic morphological changes in both strains. CONCLUSION: HA significantly inhibits the proliferation of the two cell lines, arrests the cell cycle in G1 phase and plays a more important role in Karpas299 cells, which may be related to the expression of tyrosine kinase receptor ALK protein.