【摘 要】
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Hyaluronidases (hyalases) from bovine testes and leeches were used as “standard”. Constituents of crude snake venoms-hyalases were separated by size exclusio
【机 构】
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公安部物证鉴定中心!北京100038,Dept.ofChemistryFacultyofScience!ChulalongkornUniv.,Bangkok,Tailand,Div.ofNeoplas
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Hyaluronidases (hyalases) from bovine testes and leeches were used as “standard”. Constituents of crude snake venoms-hyalases were separated by size exclusion HPLC. Molecular masses of hyalases from snake venoms, determined by on-line capillary HPLC-mass spectrometry with electrospray ionization, were: Naja melanoleuca (black cobra) 49377Da, Naja Kaothia(Thai cobra)60265Da, Sepedon hemachatus (ringhals cobra) 49722Da. Mass determinations were repeatable within 0.03%.
Hyaluronidases (hyalases) from bovine testes and leeches were used as “standard ”. Constituents of crude snake venoms-hyalases were separated by size exclusion HPLC. Molecular masses of hyalases from snake venoms, determined by on-line capillary HPLC-mass spectrometry with electrospray ionization, were: Naja melanoleuca (black cobra) 49377Da, Naja Kaothia (Thai cobra) 60265Da, Sepedon hemachatus (ringhals cobra) 49722Da. Mass determinations were repeatable within 0.03%.
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