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采用细菌通用引物和古细菌引物对青海油砂山油田178井本源微生物样品DNA进行扩增,构建基因文库,克隆和DNA序列分析。将测序结果与基因银行进行比对结果表明,油砂山样品中有大量海杆菌属和甲胞菌属,样品中还发现存在产甲烷古菌及一些不可培养的其它古菌类群。它们的功能和多样性有待于深入研究,以便进一步筛选用于提高该油田原油采收率的采油功能菌群。
Genomic DNA, clone and DNA sequence were amplified by PCR using universal primers and archaeal primers. The results of sequencing and gene bank comparison results show that there are a large number of oil sands mountain samples of genus and genus Kaidi genus, the sample also found the presence of methane-producing archaea and some other uncultured archaeal groups. Their function and diversity remain to be further explored in order to further screen for oil-producing functional microflora for enhanced oil recovery in the field.