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】采用16种不同的引物对3株典型的稻瘟病菌(Magnaporthegrisea)菌株ZA1,ZG1和M1进行了RAPD分析。结果表明,从杂草马塘与水稻分离的菌株,基因组的差异很大,很容易通过RAPD鉴别这两种不同来源的稻瘟病菌菌株。自水稻分离的菌株,RAPD扩增产物相似性较高,但仍然能够检测出不同生理小种DNA带型的差异,证明RAPD可用于稻瘟病菌生理小种的快速鉴别。
Three typical Magnaporthe grisea strains ZA1, ZG1 and M1 were analyzed by RAPD using 16 different primers. The results showed that there were great differences in the genomes of the strains isolated from weeds of Matsui and rice. It is easy to identify these two strains of Magnaporthe grisea via RAPD. RAPD amplified products from rice isolates have high similarity, but they still can detect the differences in DNA bands of different races. It is proved that RAPD can be used for the rapid identification of physiological races of M. grisea.