论文部分内容阅读
目的观察多巴胺D1类受体对胰岛素受体介导的血管平滑肌细胞增殖的影响。方法本研究以A10细胞为研究对象,观察刺激D1类受体对胰岛素促增殖作用的影响,并用免疫印迹研究D1类受体影响胰岛素作用的机制。细胞增殖作用采用[3H]胸腺嘧啶核苷(3H-TdR)掺入量表示。结果胰岛素可促进A10细胞的增殖,该作用呈现浓度依赖性的。D1类受体激动剂Fenoldopam本身对A10细胞无增殖影响,但Fenoldopam通过D1类受体可完全阻断胰岛素介导的血管平滑肌细胞增殖作用。免疫印迹显示刺激D1类受体可降低胰岛素受体的蛋白表达,提示该机制可能参与了D1类受体对胰岛素受体作用的过程。结论D1类受体对胰岛素受体介导的血管平滑肌细胞增殖具有抑制作用,该作用可能在高血压的发生发展中发挥一定作用。
Objective To observe the effects of dopamine D1 receptors on insulin receptor mediated vascular smooth muscle cell proliferation. Methods The aim of this study was to investigate the effect of stimulating D1 receptors on insulin-stimulated proliferation in A10 cells and to investigate the mechanism by which D1 receptors affect insulin action by immunoblotting. Cell proliferation was measured using [3H] thymidine incorporation (3H-TdR). Results Insulin promoted A10 cell proliferation in a concentration-dependent manner. The D1 receptor agonist Fenoldopam itself has no effect on proliferation of A10 cells, but Fenoldopam completely blocks insulin-mediated proliferation of vascular smooth muscle cells via D1 receptors. Immunoblotting showed that stimulation of D1 receptors decreased the expression of insulin receptor, suggesting that this mechanism may be involved in the action of D1 receptors on insulin receptor. Conclusion D1 receptors have inhibitory effects on insulin receptor-mediated proliferation of vascular smooth muscle cells, which may play a role in the development of hypertension.