论文部分内容阅读
目的 探讨慢性乙型病毒性肝炎 (CHB)患者血清病毒载量与IFN -γ和IL - 4的关系 ,为CHB治疗提供理论依据。方法 荧光定量聚合酶链反应 (FQ -PCR)检测HBVDNA含量和酶联免疫吸附法 (ELISA)检测IFN -γ和IL - 4含量。按HBVDNA含量 (拷贝 /ml)把CHB患者分为高病毒载量组 (≥ 10 7/ml) ,中载量组 (10 6 ～10 5/ml)和低载量组 (<10 5/ml)。结果 CHB患者与正常对照组比较 :IFN -γ显著降低 (t =4 2 0 ,P <0 0 1) ,IL- 4显著增高 (t =6 36 ,P <0 0 1)。高、中、低载量组之间比较 :IFN -γ、IL - 4含量有显著差异 (分别F =3 6 5 ,P <0 0 5 ,F =4 89,P <0 0 5 )。IFN -γ在高载量组最低 ,低载量组最高 ;IL - 4在低载量组最低 ,高载量组最高。结论 CHB患者存在一定程度TH1/TH2型细胞的失平衡 ,以TH2型细胞因子表达占优势 ,并随着病毒载量增高而加重。因此 ,调节TH1/TH2型细胞平衡有助于清除HBV和治疗CHB。
Objective To investigate the relationship between serum viral load and IFN-γ and IL-4 in patients with chronic hepatitis B (CHB) and provide a theoretical basis for CHB treatment. Methods Fluorescent quantitative polymerase chain reaction (FQ-PCR) detection of HBVDNA content and enzyme-linked immunosorbent assay (ELISA) detection of IFN-γ and IL-4 content. Patients with CHB were divided into high viral load group (≥10 7 / ml), medium load group (106 ~ 105 / ml) and low load group (<105 / ml) according to HBVDNA content ). Results Compared with the normal control group, IFN-γ was significantly lower in CHB patients (t = 420, P <0.01) and IL-4 was significantly higher (t = 6 36, P <0.01). The levels of IFN-γ and IL-4 were significantly different among high, medium and low-load groups (F = 365, P <0 05, F = 4 89, P 0 05, respectively). IFN-γ was lowest in high-load group and highest in low-load group; IL-4 was the lowest in low-load group and highest in high-load group. Conclusions There is some imbalance of TH1 / TH2 cells in CHB patients, which predominates in the expression of TH2 cytokines and aggravates with the increase of viral load. Therefore, regulation of TH1 / TH2 cell balance helps to clear HBV and treat CHB.