本文用DEAE纤维素离子交换层析、ConA—Sepharose亲和层析、Sephadex G-100凝胶过滤等技术,分离纯化妊娠3～6周正常早孕妇女静脉血混合血清中早孕因子(Early Pregnancy Factor,EPF).以花结抑制实验检测各阶段层析物中EPF活性,以放射免疫法测定hCG含量.结果表明,经凝胶过滤后,提取物中hCG均为阴性(<3.1ng/ml),高EPF活性部分经SDS-聚丙烯酰胺凝胶电泳显示,分子量分别为24KD.和41KD_α。 In this paper, DEAE cellulose ion exchange chromatography, ConA-Sepharose affinity chromatography, Sephadex G-100 gel filtration and other techniques, isolation and purification of pregnant women 3 to 6 weeks of normal early pregnancy women venous blood mixed serum early pregnancy factor (Early Pregnancy Factor, (EPF) was used to detect the activity of EPF in each stage of the chromatin, and the content of hCG was determined by radioimmunoassay.The results showed that the hCG in the extract was negative (<3.1ng / ml) after gel filtration, The fraction of high EPF activity was shown by SDS-polyacrylamide gel electrophoresis with molecular weights of 24 KD and 41 KD_α, respectively.