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具有良好的生物相容性且生物可降解天然多糖,一直作为药物传递体系,具有如下优点:可延长药物的生物半衰期,减轻药物的毒副作用.本实验旨在制备一种果胶基载5-氟脲嘧啶(5-Fluorouracil,5-FU)纳米粒子(P-5-FU)载药系统,探讨果胶基纳米载药体系本身带有大量的半乳糖残基这种天然靶头对人肝癌细胞HepG2的靶向效果.MTT法测定载药果胶基纳米粒子对HepG2和A549细胞的增殖抑制作用,MTT结果显示P-5-FU对HepG2细胞的增殖具有明显抑制作用,呈剂量依赖性,且作用较5-FU强;而P-5-FU对A549细胞增殖亦有明显抑制作用,呈剂量依赖性,但与5-FU相比无明显差别;高效液相色谱法(HPLC)对两种细胞对载药纳米粒子的摄取情况和靶向性进行了测定.细胞摄取结果显示HepG2细胞对P-5-FU的摄取量较5-FU明显增多,而A549细胞对P-5-FU和5-FU的摄取量没有明显的差别.半乳糖饱和ASGPR结合位点后两种细胞对P-5-FU和5-FU的摄取量都没有明显的差别.结果表明果胶基纳米载药粒子可特异性靶向高表达的细胞.
Has good biocompatibility and biodegradable natural polysaccharide, has been used as a drug delivery system, has the following advantages: can extend the biological half-life of drugs and reduce drug side effects.This experiment is to prepare a pectin-based 5- Fluorouracil (5-FU) Nanoparticles (P-5-FU) Drug-Delivery System To Explore The Pomegranate Nanoparticle System Contains Large Quantities of Galactose Residues This Natural Target Against Human Liver Cancer MTT assay showed that P-5-FU had a significant inhibitory effect on the proliferation of HepG2 cells in a dose-dependent manner, and the proliferation of HepG2 cells was inhibited by MTT assay. P-5-FU had a significant inhibitory effect on the proliferation of A549 cells in a dose-dependent manner, but there was no significant difference compared with 5-FU. The inhibitory effect of high performance liquid chromatography (HPLC) The uptake and targeting of drug-loaded nanoparticles were determined by cell-based assay.The uptake of P-5-FU into HepG2 cells was significantly higher than that of 5-FU, while the uptake of P-5-FU by A549 cells There was no significant difference in the uptake of 5-FU between the two cell lines after the galactose-saturated ASGPR binding sites on P-5-F U and 5-FU showed no significant difference.The results showed that pectin-based nanoparticles can specifically target highly expressed cells.