论文部分内容阅读
放射免疫是一种测定技术,应用日益广泛。由于此技术具有许多特点,已成为当今常用的超微量分析方法之一。为进行《胰岛索抗原性的研究》及《高纯度低抗原性胰岛素制剂的研究)等科研项目,自1982年开始,我室陆续建立了猪胰岛素、前胰岛素、胰高糖素和胰多肽的放射免疫分析法,并应用于检测胰岛素制剂中各种激素的含量。兹简介于下供参考。一、放射免疫分析的原理和条件放射免疫分析的基本原理是竞争性结合(Competitive binding)或竞争性抑制(Competitive inhibition),也称为置换(Displaccment)。本法是在试管内免疫反应系统中,引入放射性核素标记抗原,它与原型抗原(未
Radioimmunoassay is a measurement technique that is used more and more widely. Due to the many features of this technology, it has become one of the most commonly used methods of ultra-micro analysis. In order to carry out the research on the antigenicity of pancreatic islets and the research on the high purity and low antigenic insulin preparations, since 1982, we have successively established the porcine insulin, proinsulin, glucagon and pancreatic polypeptide Radioimmunoassay, and used to detect the content of various hormones in insulin preparations. Hereby for reference below. First, the principles and conditions of radioimmunoassay The basic principle of radioimmunoassay is competitive binding (Competitive binding) or Competitive inhibition (Competitive inhibition), also known as displacement (Displaccment). This method is in vitro immune response system, the introduction of radionuclide labeled antigen, which is not associated with the prototype antigen