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目的研究促性腺激素释放激素受体(GnRH-R)在子宫腺肌病在位内膜组织中的表达,探讨GnRH-R与子宫腺肌病的关系,为临床治疗子宫腺肌病提供理论依据。方法分离腺上皮细胞及间质细胞进行体外培养,应用免疫组织化学的方法检测上述细胞中GnRH-R的表达,分析其在子宫腺肌病的表达。结果Gn-RH-R在子宫腺肌病在位内膜腺上皮细胞100%表达,增殖期和分泌期表达差异无统计学意义(P>0.05),基质细胞无表达。结论GnRH-R在子宫腺肌病在位内膜腺上皮细胞有表达,在基质细胞无表达。
Objective To study the expression of gonadotropin-releasing hormone receptor (GnRH-R) in eutopic endometrium of adenomyosis and to explore the relationship between GnRH-R and adenomyosis and to provide a theoretical basis for clinical treatment of adenomyosis . Methods Glandular epithelial cells and stromal cells were isolated and cultured in vitro. The expression of GnRH-R was detected by immunohistochemistry and the expression of GnRH-R in adenomyosis was analyzed. Results The expression of Gn-RH-R in the eutopic endometrial glandular epithelial cells was 100%, and there was no significant difference between proliferative phase and secretory phase (P> 0.05). No expression was found in stromal cells. Conclusions GnRH-R is expressed in eutopic endometrial glandular epithelial cells in adenomyosis and non-expressed in stromal cells.