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本室已经建立了MHC不相合单份鼠胎血同种移植的小鼠模型。基于脐血造血干细胞的免疫学特性及临床实践 ,我们认为脐血移植成功的关键除了选择尽可能相合的供者外 ,保证移植细胞的数量更为重要。因此 ,为了提高移植干细胞的数量 ,本研究在前期工作的基础上 ,探讨MHC不相合的双份鼠胎血混合移植的可能性。研究结果表明 ,混合移植组 4 0只受鼠中有 2 6只在 6 0天的观察期内存活下来 ;应用PCR及流式细胞术均检测到受鼠体内有混合嵌合体的形成 ;此外 ,皮肤移植试验也发现受鼠已被诱导形成对供鼠的特异性免疫耐受 ;小肠组织的病理切片显示受鼠仅表现出轻度GVHD。结论 :MHC不相合的双份鼠胎血能够同时植入 ,重建受鼠的免疫与造血系统 ,这为拓宽脐血的临床应用 ,使更多患者能够有机会接受脐血移植做了有益的尝试。
This room has been established MHC incompatible single mouse fetal blood allograft mouse model. Based on the immunological characteristics of umbilical cord blood hematopoietic stem cells and clinical practice, we believe that the key to the success of umbilical cord blood transplantation is to ensure that the number of transplanted cells is more important than selecting donors as compatible as possible. Therefore, in order to improve the number of transplanted stem cells, this study based on the previous work, to explore the MHC incompatible twin mouse fetal blood mixed transplantation possibilities. The results showed that 26 of the 40 mice in the mixed transplantation group survived the observation period of 60 days. The formation of mixed chimerism was detected by PCR and flow cytometry. In addition, Skin grafts also found that the recipient had been induced to develop specific immune tolerance to the donor; pathological sections of the intestinal tissue showed only mild GVHD in the recipient. Conclusions: MHC-incompatible twins fetal blood can be implanted at the same time to reconstruct the immune and hematopoietic system of the recipient mice. This is a beneficial attempt to broaden the clinical application of cord blood and make more patients have the opportunity to receive cord blood transplantation .